Covaris shearing manual

DNA/RNA Shearing for NGS Nucleic acid fragmentation is a crucial first step in the Next-Gen Sequencing workflow. While there are a variety of methods available, mechanical DNA shearing powered by AFA-energetics® remains the method of choice for . E Focused-ultrasonicator. Treatment System. Bench-top; high-intensity acoustic transducer, temperature monitoring device, circulation pump, water bath with safety enclosure. Treatment Power. Watts Average Power. Dimensions. 24” (width) x 30” (depth) x 19” (height) (61cm x 76cm x 48cm) Weight. Approximately lbs (50 Kg). dsDNA shearing conditions Therefore, a “fast” protocol may be introducing a thermal -bias to the desired random fragmentation process wide dynamic range of Covaris AFA. 0 1, 1, 1, 1, 0 1, Base Pairs. Time (seconds) DNA shearing. isothermal conditions. Covaris, Inc.

Covaris ™ Part Number: Rev D. 7 | Page Date: 03 Jun Concentration independent bp DNA shearing. Figure 4 - Agilent Bioanalyzer electropherogram (12k chip) Different mass of lambda DNA (in μl TE buffer) sheared in microTUBE operating conditions on page 1. Concentration independent 1 bp DNA shearing. Overview. This kit allows users to routinely verify the performance of their Covaris Focused-ultrasonicator. The kit may be used for periodic quality assurance or used when optimizing methods. The kit contains a Reference sample of pre-fragmented genomic DNA, and a Test sample of un-fragmented genomic DNA. The kit contains sufficient material. E Focused-ultrasonicator. Treatment System. Bench-top; high-intensity acoustic transducer, temperature monitoring device, circulation pump, water bath with safety enclosure. Treatment Power. Watts Average Power. Dimensions. 24” (width) x 30” (depth) x 19” (height) (61cm x 76cm x 48cm) Weight. Approximately lbs (50 Kg).

This Quick Guide provides DNA Shearing protocols for the Covaris AFA-TUBE, microTUBE, and miniTUBE Refer to the instrument manual for complete setup. Typically, Covaris creates DNA shearing protocol guidelines to a target base pair of +/- 15% with a %CV < 10% as analyzed using the Agilent, Bioanalyzer High. This Quick Guide provides DNA Shearing protocols for the Covaris microTUBE and miniTUBE consumables Refer to the instrument manual for complete setup.